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[尿液分析外部质量评估计划(PEECU)的第二次两年期评估]

[The second biennial of the External Quality Evaluation Program in Urinalysis (PEECU)].

作者信息

Loría Alvar, Mejía Myrna, Granados Lucía, Hernández Erico

机构信息

Unidad de Epidemiología Clínica, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán Vasco de Quiroga 15, Tlalpan, 14000 México, D.F.

出版信息

Rev Invest Clin. 2002 May-Jun;54(3):226-30.

Abstract

OBJECTIVE

To characterize the participation of clinical laboratories in the second biennial of an external quality assurance scheme for urinalysis in Mexico.

METHODS

The participants received 32 control samples in 8 shipments of 4 controls each during the biennial 1999-2000. The controls were in the form of tablets to be dissolved in 12 mL of water to mimic urine. The participants performed dipstick assays of pH, specific gravity (SG) and 8 analytes (proteins, glucose, ketones, blood, bilirubin, nitrites, urobilinogen, leukocytes) in the controls. Expected control values were assigned by 10-15 replicate automated assays using dipsticks and apparatus of a single provider (Bayer). Participants' results were transformed to scores ranging from zero (perfect result) to 400 (worst possible). Score differences were analyzed using nonparametric methods (Kruskal-Wallis and Mann-Whitney).

RESULTS

The best results were seen for nitrites, ketones and proteins (mean global scores = 3-5) and the worst for SG (score = 45) with intermediate scores of 10-13 for the other six.

CONCLUSIONS

The scores improved for the second biennial from a grand mean of 17 in the first significantly down to 12 in the second. The number of participants has been increasing yearly and there has been a decrease of participants who do not report at least 3 out of 4 yearly shipments. We confirmed that automated readings yield better scores than visual readings.

摘要

目的

描述墨西哥临床实验室参与尿分析外部质量保证计划第二个双年度的情况。

方法

在1999 - 2000年双年度期间,参与者分8批收到32个对照样本,每批4个对照样本。对照样本为片剂形式,需溶解于12毫升水中以模拟尿液。参与者对对照样本进行试纸条检测,测定pH值、比重(SG)以及8种分析物(蛋白质、葡萄糖、酮体、血液、胆红素、亚硝酸盐、尿胆原、白细胞)。预期对照值通过使用单一供应商(拜耳)的试纸条和仪器进行10 - 15次重复自动检测来确定。将参与者的结果转换为从0(完美结果)到400(最差可能结果)的分数。使用非参数方法(Kruskal - Wallis和Mann - Whitney)分析分数差异。

结果

亚硝酸盐、酮体和蛋白质的检测结果最佳(总体平均分数 = 3 - 5),比重检测结果最差(分数 = 45),其他六种分析物的分数为10 - 13,处于中间水平。

结论

第二个双年度的分数从第一个双年度的总体平均17分显著降至12分。参与者数量逐年增加,未报告至少4次年度批次中3次结果的参与者数量有所减少。我们证实自动读数的分数优于目视读数。

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