Tomono T, Murokawa H, Minegishi K, Yamanaka R, Lizuka H Y, Miyamoto M, Satoh S, Nakahira S, Murozuka T, Emura H, Doi Y, Mine H, Yokoyama S, Ohnuma H, Tanaka T, Yoshikawa A, Nishioka K
JRC Headquarter, Blood Services Department.
Dev Biol (Basel). 2002;108:29-39.
The first nationwide nucleic acid amplification testing (NAT) for hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus type 1 (HIV-1) of voluntarily donated blood after serological pre-screening and before release of cellular components and plasma for fractionation was implemented by the Japanese Red Cross Blood Transfusion Services. The NAT screening assay using multiplex reagent is time-saving, cost effective, and labour-saving procedure for all blood and blood products including short-shelf life platelets. During the 50-mini-pool NAT screening of serologically negative donations (February 1, 2001-April 30, 2001), we were able to screen out 112 HBV-positive, 25 HCV-positive, and 4 HIV-1 positive units from blood and blood components.
日本红十字输血服务机构实施了首个全国性的核酸扩增检测（NAT），用于对血清学初筛后、细胞成分和血浆分馏放行前的无偿献血进行乙型肝炎病毒（HBV）、丙型肝炎病毒（HCV）和1型人类免疫缺陷病毒（HIV-1）检测。使用多重试剂的NAT筛查检测对于所有血液和血液制品（包括保质期短的血小板）而言，是一种节省时间、成本效益高且省力的程序。在对血清学阴性献血进行50分钟混合NAT筛查期间（2001年2月1日至2001年4月30日），我们从血液和血液成分中筛查出112个HBV阳性单位、25个HCV阳性单位和4个HIV-1阳性单位。
