一种测量细菌颗粒电导率的简单方法。
A simple method for the measurement of bacterial particle conductivities.
作者信息
Yunus Zalini, Mason Vincent, Verduzco-Luque Cynthia E, Markx Gerard H
机构信息
Department of Chemical Engineering, UMIST, PO Box 88, Sackville Street, Manchester M60 1QD, UK.
出版信息
J Microbiol Methods. 2002 Nov;51(3):401-6. doi: 10.1016/s0167-7012(02)00129-x.
A method was developed for the measurement of the bacterial particle conductivity, based on the measurement of the conductivity of a bacterial cell suspension sigma(s) and the suspending medium sigma(m). A line plotted through sigma(s) - sigma(m) versus sigma(m) crosses the x-axis at sigma(m) = sigma(p), independent of the bacterial cell concentration. The method does not require anything more complex than a centrifuge and a conductivity meter. Knowledge of the bacterial particle conductivity is of importance in, for example, the dielectrophoretic separation, manipulation and trapping of bacterial cells, as well as the study of their physiological state.
基于对细菌细胞悬浮液电导率σ(s)和悬浮介质电导率σ(m)的测量，开发了一种测量细菌颗粒电导率的方法。绘制一条通过σ(s) - σ(m)与σ(m)关系的直线，该直线在σ(m) = σ(p)时与x轴相交，与细菌细胞浓度无关。该方法所需的不过是一台离心机和一台电导率仪。例如，了解细菌颗粒电导率对于细菌细胞的介电电泳分离、操控和捕获以及对其生理状态的研究都很重要。
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