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Putrescine Channels in the Plasma Membrane of Arabidopsis thaliana.

作者信息

Giromini L., Paina A., Cerana R., Colombo R.

机构信息

Dipartimento di Biologia, Universita degli Studi di Milano, via Celoria, 26, I-20133 Milano, Italy.

出版信息

Plant Physiol. 1994 Jul;105(3):921-926. doi: 10.1104/pp.105.3.921.

Abstract

The patch-clamp technique was used in the whole-cell configuration to study plasma membrane channels permeable to the diamine putrescine in protoplasts isolated from cultured cells of Arabidopsis thaliana L. Under our experimental conditions, no channels selectively mediating putrescine influx were observed. Inward K+ channels showed a low permeability to putrescine, the permeability ratio of putrescine relative to K+ being around 0.1. Further characterization of the previously identified outward channels mediating putrescine efflux (R. Colombo, R. Cerana, N. Bagni [1992] Biochem Biophys Res Commun 182: 1187-1192) indicated that their activity was regulated by the overall ion concentration of the external medium.

摘要

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