猪源弓形虫致密颗粒抗原（GRA6）基因的克隆

Clone of the gene of the dense granule antigen (GRA6) of the Toxoplasma gondii pig strain.

作者信息

Yu Chuanxin, Yin Xeren, Zhu YinChangj

机构信息

Jiangsu Institute of Parasitic Diseases, PR China.

出版信息

Southeast Asian J Trop Med Public Health. 2002 Jun;33(2):235-40.

PMID:12236419

Abstract

The gene of GRA6 of Toxoplasma gondii pig strain was cloned; and the value of recombinant GRA6 as a diagnostic antigen of toxoplasmosis in China was investigated. A pair of primers was synthesized according to the DNA sequence of GRA6 of T. gondii RH strain, and the DNA of pig strain tachyzoites was prepared. A single, specific DNA fragment was successfully amplified from the genomic DNA of tachyzoites of T. gondii. This gene fragment was cloned into the plasmid pUC19 to form the recombinant. The recombinant plasmid was purified and the foreign DNA fragment was sequenced. The result of DNA sequencing showed that the open reading frame of GRA6 is composed of 693 base pairs and encodes 230 amino acids and is 100% homologous with the sequence of GRA6 of T. gondii RH strain.

摘要

克隆了猪源弓形虫GRA6基因；并研究了重组GRA6作为中国弓形虫病诊断抗原的价值。根据弓形虫RH株GRA6的DNA序列合成一对引物，制备猪株速殖子的DNA。成功从弓形虫速殖子基因组DNA中扩增出单一、特异性的DNA片段。将该基因片段克隆到质粒pUC19中形成重组体。纯化重组质粒并对外源DNA片段进行测序。DNA测序结果表明，GRA6的开放阅读框由693个碱基对组成，编码230个氨基酸，与弓形虫RH株GRA6序列100%同源。