Fukami Takeshi, Satoh Hitoshi, Fujita Eriko, Maruyama Tomoko, Fukuhara Hiroshi, Kuramochi Masami, Takamoto Shinichi, Momoi Takashi, Murakami Yoshinori
Tumor Suppression & Functional Genomics Project, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo 104-0045, Japan.
Gene. 2002 Jul 24;295(1):7-12. doi: 10.1016/s0378-1119(02)00835-1.
We have recently identified the TSLC1 gene as a novel tumor suppressor in human non-small lung cancer on chromosome 11q23.2. TSLC1 encodes a membrane glycoprotein showing significant homology with immunoglobulin superfamily molecules. Here, we report the isolation of a mouse orthologous gene, Tslc1. The Tslc1 cDNA contains a single open reading frame of 1335 bp encoding a putative protein of 445 amino acids, and its expression was detected in all tissues examined. The Tslc1 gene is mapped on mouse chromosome 9, a synteny of human chromosome 11q, and is composed of ten exons, the exon-intron junctions being highly conserved between human and mouse. The predicted amino acids of mouse Tslc1 display 98% identity with that of human TSLC1. Furthermore, data base analysis indicates that the amino acid sequences corresponding to the cytoplasmic domain of Tslc1 are identical in five mammals and highly conserved in vertebrates, suggesting an important role of Tslc1 in normal cell-cell interaction.
我们最近确定TSLC1基因为人类11q23.2染色体上非小细胞肺癌中的一种新型肿瘤抑制基因。TSLC1编码一种与免疫球蛋白超家族分子具有显著同源性的膜糖蛋白。在此，我们报告小鼠直系同源基因Tslc1的分离。Tslc1 cDNA包含一个1335 bp的单一开放阅读框，编码一个445个氨基酸的推定蛋白，并且在所有检测的组织中均检测到其表达。Tslc1基因定位于小鼠9号染色体，与人11号染色体q区存在同线性，由十个外显子组成，外显子-内含子连接在人和小鼠之间高度保守。小鼠Tslc1的预测氨基酸与人类TSLC1的预测氨基酸具有98%的同一性。此外，数据库分析表明，与Tslc1细胞质结构域对应的氨基酸序列在五种哺乳动物中相同，在脊椎动物中高度保守，提示Tslc1在正常细胞间相互作用中起重要作用。
