鼓腹咝蝰毒液对动物血浆蛋白的沉淀作用。

Precipitation of animal plasma proteins by puff-adder venom.

Hattingh J, Joos-Vandewalle P W, Willemse G T

Department of General Physiology, University of the Witwatersrand, Johannesburg, South Africa.

Comp Biochem Physiol B. 1979;63(4):557-9. doi: 10.1016/0305-0491(79)90062-2.

Abstract

Plasma and serum samples obtained from various animals never previously exposed to snakes or snake venom were diffused against different concentrations of puff-adder, Bitis arietans, venom using the double immunodiffusion technique. 2. Depending upon venom concentration, two precipitin arcs could be produced in the case of all plasma samples used. No serum samples showed any arcs except pigeon serum, where one precipitin line was observed. 3. By altering the concentration of snake venom between 1% and 10% when immunodiffusing against plasma a change in position of the precipitin lines was observed and also the disappearance of one or both of the two bands at higher concentrations. This indicates that the arcs observed are in all probability due to precipitation of plasma protein fractions. 4. Previous results indicated that one of the two bands observed when diffusing venom against plasma was due to the precipitation of fibrinogen. By diffusing snake venom against heparin we have now shown that the second band involves this molecule and is not due to another coagulation factor as was suggested previously.

摘要

使用双向免疫扩散技术，将从未接触过蛇或蛇毒的各种动物的血浆和血清样本，与不同浓度的鼓腹咝蝰（Bitis arietans）毒液进行扩散试验。2. 根据毒液浓度，所有使用的血浆样本都能产生两条沉淀弧。除了鸽血清观察到一条沉淀线外，没有血清样本显示出任何弧。3. 在与血浆进行免疫扩散时，将蛇毒浓度在1%至10%之间改变，观察到沉淀线位置的变化，并且在较高浓度下两条带中的一条或两条消失。这表明观察到的弧很可能是由于血浆蛋白组分的沉淀。4. 先前的结果表明，毒液与血浆扩散时观察到的两条带之一是由于纤维蛋白原的沉淀。通过将蛇毒与肝素进行扩散试验，我们现在表明第二条带涉及该分子，而不是如先前所认为的由于另一种凝血因子。