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[利用土壤的伽马射线辐照分析微生物群落状态]

[Use of gamma-irradiation of soil for analyzing the state of microbial coeneses].

作者信息

Shcherbakova T A, Nikitin D I, Volkovskaia N G

出版信息

Mikrobiologiia. 1975 Mar-Apr;44(2):325-9.

PMID:1226145
Abstract

Increasing doses of gamma-irradiation eliminate some physiological and ecologo-trophical groups from microbial cenoses in soil. Soil fungi are least resistant to the action of gamma-irradiation (do not grow on agarized soil (AS) at a dose of 0.5 Mrad). Actinomycetes are more resistant (do not grow on AS at a dose of 0.75 Mrad). Bacteria are most resistant to irradiation. Asporogenous bacteria do not grow on agarized soil at a dose of 1.25 Mrad. Sporogenous bacteria survive at higher doses (1.75 Mrad). The method of gamma-irradiation permits to accomplish differential analysis of ecological groups of soil microflora and experimental synthesis of microbial associations. The effect of gamma-irradiation on soil enzymes is weak compared with autoclaving. gamma-Irradiated soils may serve as a substrate for model experiments during analysis of microbial associations.

摘要

增加剂量的γ射线辐照可从土壤中的微生物群落中消除一些生理和生态营养类群。土壤真菌对γ射线辐照的抵抗力最弱(在剂量为0.5兆拉德时,不在琼脂化土壤(AS)上生长)。放线菌更具抗性(在剂量为0.75兆拉德时,不在AS上生长)。细菌对辐照的抵抗力最强。无芽孢细菌在剂量为1.25兆拉德时不在琼脂化土壤上生长。芽孢细菌在更高剂量(1.75兆拉德)下存活。γ射线辐照方法可对土壤微生物区系的生态类群进行差异分析,并实现微生物群落的实验合成。与高压灭菌相比,γ射线辐照对土壤酶的影响较弱。γ射线辐照过的土壤可作为分析微生物群落时模型实验的底物。

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