Zaslavsky Boris, Uthus Eric O
FDA/CBER HFM-217, Rockville, MD 20852-1448, USA.
Biol Trace Elem Res. 2002 Aug;88(2):165-83. doi: 10.1385/BTER:88:2:165.
An analysis of the interaction between dietary iron (Fe) and zinc (Zn) was performed by using data from Sprague-Dawley rats in a 5 x 4 fully crossed factorially arranged experiment. The concentrations of 9 trace elements from the liver and 10 from the heart were determined and subjected to diverse statistical analyses and were classified by their response to the interaction between dietary Fe and Zn. The interaction was studied by using analysis of variance (ANOVA), discriminant analysis, and logistic regression to determine the direction of interaction; that is, did dietary Fe affect dietary Zn or did dietary Zn affect dietary Fe? The use of discriminant analysis allowed for using multiple parameters (rather than a single parameter) to determine possible interactions between Fe and Zn. Thus, two main levels of interaction were studied: the separate response of each tissue mineral and the response of some grouped minerals. The responses studied were the effect of dietary Zn on tissue trace element parameters, the effect of dietary Fe on the parameters, the effect of dietary Zn on combined (grouped) parameters, and the effect of dietary Fe on combined parameters. As determined by ANOVA, only three individual trace elements--liver Fe, Cu, and Mo--were significantly affected by the interaction between Fe and Zn. However, a broader interaction between Fe and Zn is revealed when groups of, rather than single, trace elements arestudied. For example, an interaction between dietary Fe and Zn affects the weighted linear combination of heart Ca, Cu, K, Mg, Mn, P, and Zn. This article presents the hypothesis that grouped parameters may be useful as status indicators. The complete dataset can be found at http://www.gfhnrc.ars. usda.gov/fezn.
通过使用来自斯普拉格-道利大鼠的5×4完全交叉析因实验数据，对膳食铁（Fe）和锌（Zn）之间的相互作用进行了分析。测定了肝脏中的9种微量元素和心脏中的10种微量元素的浓度，并进行了各种统计分析，并根据它们对膳食铁和锌之间相互作用的反应进行了分类。通过方差分析（ANOVA）、判别分析和逻辑回归来研究这种相互作用，以确定相互作用的方向；也就是说，膳食铁是否影响膳食锌，或者膳食锌是否影响膳食铁？判别分析的使用允许使用多个参数（而不是单个参数）来确定铁和锌之间可能的相互作用。因此，研究了两个主要的相互作用水平：每个组织矿物质的单独反应和一些分组矿物质的反应。所研究的反应包括膳食锌对组织微量元素参数的影响、膳食铁对这些参数的影响、膳食锌对组合（分组）参数的影响以及膳食铁对组合参数的影响。通过方差分析确定，只有三种单个微量元素——肝脏中的铁、铜和钼——受到铁和锌之间相互作用的显著影响。然而，当研究的是微量元素组而不是单个微量元素时，铁和锌之间存在更广泛的相互作用。例如，膳食铁和锌之间的相互作用会影响心脏中钙、铜、钾、镁、锰、磷和锌的加权线性组合。本文提出了分组参数可能作为状态指标有用的假设。完整的数据集可在http://www.gfhnrc.ars.usda.gov/fezn上找到。
