胆固醇对精浆蛋白PDC-109与磷脂酰胆碱膜相互作用的影响。

Effect of cholesterol on the interaction of seminal plasma protein, PDC-109 with phosphatidylcholine membranes.

作者信息

Swamy Musti J, Marsh D, Anbazhagan V, Ramakrishnan M

机构信息

School of Chemistry, University of Hyderabad, India.

出版信息

FEBS Lett. 2002 Sep 25;528(1-3):230-4. doi: 10.1016/s0014-5793(02)03316-1.

DOI:10.1016/s0014-5793(02)03316-1

PMID:12297311

Abstract

Binding of PDC-109, the major protein of the bovine seminal plasma, to sperm plasma membrane results in an efflux of cholesterol and choline phospholipids, a necessary event before capacitation can occur. The selectivity of PDC-109 for different spin-labelled phospholipids and sterol probes in dimyristoylphosphatidylcholine (DMPC) host matrix has been characterized earlier by EPR spectroscopy [Ramakrishnan, M., Anbazhagan, V., Pratap, T.V., Marsh, D. and Swamy, M.J. (2001) Biophys. J. 81, 2215-2225]. In this report the effect of cholesterol on the interaction of PDC-109 with DMPC membranes has been investigated by spin-label EPR spectroscopy. The results indicate that the presence of cholesterol leads to an increased association of different phospholipid as well as sterol probes, thus modulating the interaction of PDC-109 with phospholipid membranes.

摘要

牛精浆中的主要蛋白质PDC - 109与精子质膜结合会导致胆固醇和胆碱磷脂外流，这是获能发生前的一个必要事件。EPR光谱法已在之前对PDC - 109在二肉豆蔻酰磷脂酰胆碱（DMPC）主体基质中对不同自旋标记磷脂和甾醇探针的选择性进行了表征[Ramakrishnan, M., Anbazhagan, V., Pratap, T.V., Marsh, D. 和 Swamy, M.J. (2001) Biophys. J. 81, 2215 - 2225]。在本报告中，通过自旋标记EPR光谱法研究了胆固醇对PDC - 109与DMPC膜相互作用的影响。结果表明，胆固醇的存在导致不同磷脂以及甾醇探针的结合增加，从而调节了PDC - 109与磷脂膜的相互作用。