Li Rong, Liu Xiao-li, Du Qing-feng, Tian Hong, Song Lan-lin, Zhang Yan, Yang Yi, Zhou Shu-yun
Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510015, China.
Di Yi Jun Yi Da Xue Xue Bao. 2002 Sep;22(9):788-90.
To elucidate the mechanism by which harringtonine (HT) induces apoptosis in K562 cell line.
By means of cell morphology, DNA gel electrophoresis and flow cytometry, we explored the action of HT on K562 cell line. Further study of the changes in bcr/abl gene expression was conducted using reverse transcriptase (RT)-PCR.
HT induced apoptosis of K562 cells at the concentrations ranging from 0.01 to 100 microg/ml, exhibiting dose- and time-dependent increase in apoptotic ratios of the cells subjected to the treatment courses of 12 to 60 h. RT-PCR showed that bcr/abl gene expression was down-regulated after K562 cells had been treated with 10 microg/ml HT.
Low concentration of HT can induce apoptosis in K562 cell line, possibly through the down-regulation of bcr/abl gene expression.
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