Stefanni S, Cerù A, Ciofi Luzzatto A, Devirgiliis Conti L
Riv Istochim Norm Patol. 1975;19(1-4):1-11.
The corneal epithelium of Triturus cristatus was studied by light and electron microscopy. It consists of three cellular layers; the superficial cells, which show numerous short microvilli, are held together by typical junctional complexes. The basal cells adhere to the basal lamina by hemidesmosomes. Furthermore all cells form with the neighboring ones interdigitations and establish with them desmosomal contacts. Secretion granules and vesicles are present throughout the epithelium, mainly in the upper layer; they are filled with an electron dense material, containing glycoproteins as suggested by the histochemical analysis. Mitoses are very frequent in the basal layer. Occasionally exfoliating cells showing signs of degeneration were observed in the superficial layer. The morphological changes occurring during epithelization of wound linearly incised, were studied by light microscopy at several time intervals and the detachement and migration of the cells was particularly investigated. Very early after wounding (5') necrotic cells and cell debris are present at the wound edges. Soon after, thin protrusions originate from surrounding undamaged cells and push through the cellular fragments sliding on the underlining connective tissue. After 2 h, the wound is completely closed by such flattened protrusions. The newly formed epithelium becomes gradually thicker (3-7 h) and the mitotic activity till now suppressed, is resumed (24 h). Studies are in progress on the modifications of the junctional complexes and on the enzymatic mechanism possibly involved in cell mobilization.
用光学显微镜和电子显微镜对欧洲冠螈的角膜上皮进行了研究。它由三层细胞组成；表层细胞有许多短微绒毛，通过典型的连接复合体连接在一起。基底细胞通过半桥粒附着于基膜。此外，所有细胞都与相邻细胞形成指状交叉，并与它们建立桥粒连接。分泌颗粒和小泡遍布上皮细胞，主要在上层；它们充满电子致密物质，组织化学分析表明其中含有糖蛋白。有丝分裂在基底层非常频繁。偶尔在表层观察到有退化迹象的脱落细胞。通过光学显微镜在几个时间间隔研究线性切开伤口上皮化过程中发生的形态变化，并特别研究了细胞的脱离和迁移。受伤后很早（5分钟），坏死细胞和细胞碎片就出现在伤口边缘。不久之后，周围未受损的细胞产生细突起，穿过在下方结缔组织上滑动的细胞碎片向前推进。2小时后，伤口被这些扁平突起完全封闭。新形成的上皮逐渐变厚（3 - 7小时），此前受到抑制的有丝分裂活性恢复（24小时）。关于连接复合体的变化以及可能参与细胞移动的酶机制的研究正在进行中。
