利用高速逆流色谱法从大花红景天中分离红景天苷

Separation of salidroside from Rhodiola crenulata by high-speed counter-current chromatography.

作者信息

Han Xiao, Zhang Tianyou, Wei Yun, Cao Xueli, Ito Yoichiro

机构信息

Beijing Research Center for Separation & Purification Technologies of Natural Products, Beijing Institute of New Technology Application, China.

出版信息

J Chromatogr A. 2002 Sep 20;971(1-2):237-41. doi: 10.1016/s0021-9673(02)01041-5.

DOI:10.1016/s0021-9673(02)01041-5

PMID:12350119

Abstract

High-speed counter-current chromatography (HSCCC) was used to purify salidroside from an extract of Rhodiola crenulata with two steps using a two-phase solvent system composed of ethyl acetate-n-butanol-water (1:4:5, v/v) in the first run and chloroform-methanol-isopropanol-water (5:6:1:4) in the second run. The method yielded 21.9 mg of salidroside from 1.216 g of the crude sample at 98% purity determined by HPLC analyses. Identification was performed by 1H NMR, 13C NMR, and MS.

摘要

采用高速逆流色谱法（HSCCC）从西藏红景天提取物中纯化红景天苷，分两步进行。第一步使用乙酸乙酯 - 正丁醇 - 水（1:4:5，v/v）组成的两相溶剂系统，第二步使用氯仿 - 甲醇 - 异丙醇 - 水（5:6:1:4）。通过高效液相色谱分析测定，该方法从1.216 g粗样品中得到了纯度为98%的21.9 mg红景天苷。通过1H NMR、13C NMR和质谱进行鉴定。