Gustafsson T, Knutsson A, Puntschart A, Kaijser L, Nordqvist A-C Sandberg, Sundberg C J, Jansson E
Department of Medical Library Sciences and Technology, Huddinge University Hospital, Karolinska Institutet, 141 86 Stockholm, Sweden.
Pflugers Arch. 2002 Sep;444(6):752-9. doi: 10.1007/s00424-002-0845-6. Epub 2002 Aug 1.
The effects of short-term exercise training on vascular endothelial growth factor (VEGF) and one of its regulatory transcription factors, the hypoxia inducible factor 1 (HIF-1) subunit, were studied in eight healthy males. Muscle and blood samples were obtained before the 1st, and 24 h after the 7th training session. VEGF and HIF-1 mRNA were analysed using RT-PCR, VEGF mRNA localization with in situ hybridization and VEGF protein with ELISA. Concurrent increases in VEGF mRNA and protein levels were observed in skeletal muscle, and the mRNA was expressed within the skeletal muscle fibres and in cells in the interstitium. These data support the idea of a pretranslational regulation of exercise-induced changes in VEGF mRNA, and indicate that increased VEGF protein expression is an early event in skeletal muscle adaptation to training. Furthermore, different cell types may act as sources for the production of angiogenic factors in response to exercise. The levels of HIF-1 mRNA subunits did not change, suggesting no change in HIF-1 mRNA transcript levels in the regulation of training-induced VEGF expression. In contrast to increased tissue VEGF expression, the arterial and femoral venous plasma levels of VEGF were decreased by training, which may indicate an exercise-induced enhancement of the peripheral uptake of VEGF.
在八名健康男性中研究了短期运动训练对血管内皮生长因子（VEGF）及其调控转录因子之一缺氧诱导因子1（HIF-1）亚基的影响。在第1次训练前以及第7次训练后24小时采集肌肉和血液样本。使用逆转录聚合酶链反应（RT-PCR）分析VEGF和HIF-1 mRNA，通过原位杂交分析VEGF mRNA定位，并用酶联免疫吸附测定法（ELISA）检测VEGF蛋白。在骨骼肌中观察到VEGF mRNA和蛋白水平同时升高，且mRNA在骨骼肌纤维内和间质细胞中表达。这些数据支持运动诱导的VEGF mRNA变化存在转录前调控的观点，并表明VEGF蛋白表达增加是骨骼肌适应训练的早期事件。此外，不同细胞类型可能作为运动后产生血管生成因子的来源。HIF-1 mRNA亚基水平未发生变化，表明在训练诱导的VEGF表达调控中HIF-1 mRNA转录水平没有改变。与组织VEGF表达增加相反，训练使动脉和股静脉血浆中的VEGF水平降低，这可能表明运动诱导了VEGF外周摄取的增强。
