乳杆菌属肠膜明串珠菌亚种肠膜明串珠菌CNRZ 1273中氨基酸转运的特性分析

Characterization of amino acid transport in the dairy strain Leuconostoc mesenteroides subsp. mesenteroides CNRZ 1273.

作者信息

Gendrot F, Foucaud-Scheunemann C, Ferchichi M, Hemme D

机构信息

Unité de Recherches Laitières et Génétique Appliquée, INRA, Jouy en Josas, France.

出版信息

Lett Appl Microbiol. 2002;35(4):291-5. doi: 10.1046/j.1472-765x.2002.01191.x.

DOI:10.1046/j.1472-765x.2002.01191.x

PMID:12358690

Abstract

AIMS

To identify and characterize amino acid transport in Leuconostoc mesenteroides.

METHODS AND RESULTS

The transport of labelled amino acids was measured in whole cells of Leuc. mesenteroides CNRZ 1273. Systems were operative under physiological conditions of growth, energy dependent and differed from peptide transport. Some of the systems were shared by several amino acids. Kinetic analysis indicated the presence of three transport systems with very high (VH), high (H) and low affinity (H) for the 11 amino acids studied. The K(t) values (micromol l(-1)) ranged from 0.088 to 0.815 (VH), 6-390 (H) and 320-4500 (L) and the V(max) values [nmol s(-1) (g dry weight)(-1)] from 0.015 to 0.8 (VH), 15-95 (H) and 90-470 (L).

CONCLUSIONS

The study showed the presence of three transport systems in Leuc. mesenteroides for all amino acids tested, some of them being shared by several amino acids.

SIGNIFICANCE AND IMPACT OF THE STUDY

The findings are discussed with reference to the growth of Leuc. mesenteroides in milk as pure or in mixed-strain culture with Lactococcus lactis.

摘要

目的

鉴定和表征肠系膜明串珠菌中的氨基酸转运。

方法与结果

在肠系膜明串珠菌CNRZ 1273的全细胞中测量标记氨基酸的转运。这些转运系统在生长的生理条件下起作用，依赖能量且与肽转运不同。一些系统为几种氨基酸所共有。动力学分析表明存在三种对所研究的11种氨基酸具有极高（VH）、高（H）和低亲和力（L）的转运系统。K(t)值（微摩尔/升）范围为0.088至0.815（VH）、6至390（H）和320至4500（L），V(max)值[nmol s(-1) (g干重)(-1)]范围为0.015至0.8（VH）、15至95（H）和90至470（L）。