雄激素对雄激素受体的体内调节作用。
In vivo modulation of androgen receptor by androgens.
作者信息
Kumar V L, Majumder P K, Kumar V
机构信息
Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110 029, India.
出版信息
Asian J Androl. 2002 Sep;4(3):229-31.
AIM
To study the effect of androgen and antiandrogen on the level of androgen receptor (AR) mRNA.
METHODS
The total RNA was extracted from the prostate and analyzed by slot blot analysis. The blots were hybridized with AR cDNA probe and 1A probe (internal control) and autoradiography was performed. The intensity of signal was measured with a densitometer and the ratio of AR RNA and 1A RNA was calculated.
RESULTS
Androgenic deprivation produced by castration decreased the weight of the prostate and increased the levels of AR mRNA. Treatment of the castrated rats with testostrone increased the weight of prostate and decreased the levels of AR mRNA. Treatment of normal rats with flutamide decreased the weight of the gland and increased the levels of AR mRNA.
CONCLUSION
Androgens produce proliferative effect on the prostate and negatively regulate the AR transcription.
目的
研究雄激素和抗雄激素对雄激素受体（AR）mRNA水平的影响。
方法
从前列腺中提取总RNA，采用狭缝杂交分析法进行分析。将杂交膜与AR cDNA探针和1A探针（内对照）杂交，然后进行放射自显影。用密度计测量信号强度，计算AR RNA与1A RNA的比值。
结果
去势导致的雄激素剥夺使前列腺重量减轻，AR mRNA水平升高。用睾酮治疗去势大鼠可增加前列腺重量，降低AR mRNA水平。用氟他胺治疗正常大鼠可使腺体重量减轻，AR mRNA水平升高。
结论
雄激素对前列腺产生增殖作用，并对AR转录起负调控作用。
Zotero 插件