一氧化氮合成参与持续血流变化后的动脉触珠蛋白表达。

Nitric oxide synthesis is involved in arterial haptoglobin expression after sustained flow changes.

作者信息

Smeets Mirjam B, Pasterkamp Gerard, Lim Sai-Kiang, Velema Evelyn, van Middelaar Ben, de Kleijn Dominique P V

机构信息

Experimental Cardiology Laboratory, University Medical Center, Heidelberglaan 100 (room G02.523), 3584 CX Utrecht, The Netherlands.

出版信息

FEBS Lett. 2002 Oct 9;529(2-3):221-4. doi: 10.1016/s0014-5793(02)03343-4.

DOI:10.1016/s0014-5793(02)03343-4

PMID:12372604

Abstract

The acute phase protein haptoglobin is highly expressed in arteries after sustained flow changes and involved in cell migration and arterial restructuring. In the liver, haptoglobin expression is mainly regulated by interleukin-6 (IL-6). In the artery, shear stress and NO influence IL-6 expression. In the present study, we demonstrate that NO synthesis is involved in the regulation of arterial haptoglobin expression after sustained flow changes. Decreased haptoglobin expression after NO inhibition coincided with decreased IL-6 levels. However, IL-6 knockout mice had normal arterial haptoglobin expression levels after sustained flow changes suggesting that other mediators may provide compensatory mechanisms for the regulation of arterial haptoglobin expression.

摘要

急性期蛋白触珠蛋白在血流持续变化后于动脉中高表达，并参与细胞迁移和动脉重构。在肝脏中，触珠蛋白的表达主要受白细胞介素-6（IL-6）调控。在动脉中，剪切应力和一氧化氮（NO）影响IL-6的表达。在本研究中，我们证明NO合成参与了血流持续变化后动脉触珠蛋白表达的调控。抑制NO后触珠蛋白表达降低与IL-6水平下降相一致。然而，IL-6基因敲除小鼠在血流持续变化后动脉触珠蛋白表达水平正常，这表明其他介质可能为动脉触珠蛋白表达的调控提供补偿机制。