Moschandreas J, Vissers M N, Wiseman S, van Putte K P, Kafatos A
Preventive Medicine and Nutrition Clinic, Department of Social Medicine, School of Medicine, University of Crete, Heraklion, Crete, Greece.
Eur J Clin Nutr. 2002 Oct;56(10):1024-9. doi: 10.1038/sj.ejcn.1601444.
To examine the effect of a low phenol olive oil and high phenol olive oil on markers of oxidation and plasma susceptibility to oxidation in normolipaemic smokers.
Randomized single-blind cross-over trial with two intervention periods.
The Medical School and University Hospital of the University of Crete, Heraklion, Crete, Greece.
Twenty-five healthy males and females completed the study.
Each intervention was of three weeks duration and intervention periods were separated by a two week washout. Seventy grams of extra virgin olive oil was supplied to each subject per day in the intervention periods. The olive oils supplied differed in their phenol content by 18.6 mg/day. Two fasting venous blood samples were taken at the end of each intervention period.
The markers of antioxidant capacity measured in fasting plasma samples (total plasma resistance to oxidation, concentrations of protein carbonyl as a marker of protein oxidation, malondialdehyde and lipid hydroperoxides as markers of lipid oxidation and the ferric reducing ability of plasma) did not differ significantly between the low and high phenol olive oil diets.
No effect of olive oil phenols on markers of oxidation in smokers was detected. It may be that the natural concentrations of phenols in olive oil are too low to produce an effect in the post-absorptive phase. Possible reasons for period effects and interactions between diet and administration period need attention to aid further cross-over trials of this kind.
Unilever Research Vlaardingen, The Netherlands.
研究低酚橄榄油和高酚橄榄油对血脂正常的吸烟者氧化指标及血浆氧化易感性的影响。
随机单盲交叉试验，有两个干预期。
希腊克里特岛伊拉克利翁市克里特大学医学院及大学医院。
25名健康男性和女性完成了该研究。
每次干预为期3周，干预期之间有2周的洗脱期。在干预期，每天向每位受试者提供70克特级初榨橄榄油。所提供的橄榄油酚含量相差18.6毫克/天。在每个干预期结束时采集两份空腹静脉血样。
在空腹血浆样本中测得的抗氧化能力指标（血浆总抗氧化能力、作为蛋白质氧化标志物的蛋白质羰基浓度、作为脂质氧化标志物的丙二醛和脂质氢过氧化物以及血浆铁还原能力）在低酚橄榄油饮食和高酚橄榄油饮食之间没有显著差异。
未检测到橄榄油酚对吸烟者氧化指标有影响。可能是橄榄油中酚的天然浓度过低，在吸收后阶段无法产生作用。周期效应以及饮食与给药期之间相互作用的可能原因需要关注，以帮助进行此类进一步的交叉试验。
荷兰联合利华研究弗拉尔丁根公司。
