Rozalski Rafal, Gackowski Daniel, Roszkowski Krzysztof, Foksinski Marek, Olinski Ryszard
Department of Clinical Biochemistry, The Ludwik Rydygier Medical University in Bydgoszcz, Karlowicza 24, 85-092 Bydgoszcz, Poland.
Cancer Epidemiol Biomarkers Prev. 2002 Oct;11(10 Pt 1):1072-5.
Using high-performance liquid chromatography prepurification/isotope dilution gas chromatography/mass spectrometry technique, we examined whether the amount of 8-hydroxyguanine and 8-hydroxy-2'-deoxyguanosine excreted into urine is higher in cancer patients with advanced-stage disease than in the control group. The control group consisted of 38 healthy subjects, and the patient group comprised 42 cancer patients suffering from metastasis of their primary tumors into the bones. We have found that the amount of the modified base (but not the nucleoside) excreted into urine is about 50% higher in cancer patients than in the control group. Because the presence of the modified base in urine may represent the primary repair product of oxidative DNA damage in vivo, our results suggest an important role of DNA glycosylases (most likely OGG1) in removal of the damage induced as a result of cancer development.
使用高效液相色谱预纯化/同位素稀释气相色谱/质谱技术，我们研究了晚期癌症患者尿液中8-羟基鸟嘌呤和8-羟基-2'-脱氧鸟苷的排泄量是否高于对照组。对照组由38名健康受试者组成，患者组包括42名原发性肿瘤已转移至骨骼的癌症患者。我们发现，癌症患者尿液中排泄的修饰碱基（而非核苷）量比对照组高约50%。由于尿液中修饰碱基的存在可能代表体内氧化性DNA损伤的主要修复产物，我们的结果表明DNA糖基化酶（很可能是OGG1）在去除癌症发展所致损伤中起重要作用。
