Rep Martijn, Dekker Henk L, Vossen Jack H, de Boer Albert D, Houterman Petra M, Speijer Dave, Back Jaap W, de Koster Chris G, Cornelissen Ben J C
Plant Pathology, Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands.
Plant Physiol. 2002 Oct;130(2):904-17. doi: 10.1104/pp.007427.
The protein content of tomato (Lycopersicon esculentum) xylem sap was found to change dramatically upon infection with the vascular wilt fungus Fusarium oxysporum. Peptide mass fingerprinting and mass spectrometric sequencing were used to identify the most abundant proteins appearing during compatible or incompatible interactions. A new member of the PR-5 family was identified that accumulated early in both types of interaction. Other pathogenesis-related proteins appeared in compatible interactions only, concomitantly with disease development. This study demonstrates the feasibility of using proteomics for the identification of known and novel proteins in xylem sap, and provides insights into plant-pathogen interactions in vascular wilt diseases.
研究发现,番茄(Lycopersicon esculentum)木质部汁液中的蛋白质含量在感染维管束枯萎病菌尖孢镰刀菌后会发生显著变化。利用肽质量指纹图谱和质谱测序技术来鉴定在亲和或非亲和互作过程中出现的最丰富的蛋白质。鉴定出了PR-5家族的一个新成员,它在两种互作类型中都早期积累。其他病程相关蛋白仅在亲和互作中出现,且与病害发展同步。本研究证明了利用蛋白质组学鉴定木质部汁液中已知和新蛋白质的可行性,并为维管束枯萎病中的植物-病原菌互作提供了见解。