大肠杆菌中DNA生物合成的能量需求。膜结合能量转换ATP酶（偶联因子）的作用。
Energy requirements for biosynthesis of DNA in Escherichia coli. Role of membrane-bound energy-transducing ATPase (coupling factor).
作者信息
Mével-Ninio M T, Valentine R C
出版信息
Biochim Biophys Acta. 1975 Mar 20;376(3):485-91. doi: 10.1016/0005-2728(75)90169-3.
A mutant of Escherichia coli missing energy-transducing ATPase and known to be defective in a variety of membrane functions from earlier studies (Yamamoto, T. H., Mével-Ninio, M. and Valentine, R. C. (1973) Biochim. Biophys. Acta 314, 267-275; Thipayathasana, P. and Valentine, R. C. (1974) Biochim. Biophys. Acta 347, 464-468; Mével-Ninio, M. and Yamamoto, T. (1974) Biochim. Biophys. Acta 357, 63-66) has been found to be blocked for anaerobic DNA synthesis. The rate of anaerobic DNA synthesis in the mutant, measured as radioactive adenine incorporation into the alkali-resistant fraction of whole cells, is about 1/6 the rate of DNA synthesis in the wild type culture under similar conditions. Addition of NO-3- or O-2 restores DNA biosynthesis in the mutant. The entry of radioactive adenine is not appreciably affected in the mutant by anaerobiosis. It is concluded that coupling factor plays a role in some step(s) of DNA biosynthesis.
已发现一种缺失能量转换型ATP酶的大肠杆菌突变体，根据早期研究（山本，T.H.，梅韦尔 - 尼尼奥，M.和瓦伦丁，R.C.（1973年）《生物化学与生物物理学报》314卷，267 - 275页；蒂帕亚塔萨纳，P.和瓦伦丁，R.C.（1974年）《生物化学与生物物理学报》347卷，464 - 468页；梅韦尔 - 尼尼奥，M.和山本，T.（1974年）《生物化学与生物物理学报》357卷，63 - 66页），该突变体在多种膜功能方面存在缺陷。以放射性腺嘌呤掺入全细胞耐碱部分来衡量，该突变体中厌氧DNA合成的速率约为相似条件下野生型培养物中DNA合成速率的1/6。添加硝酸根离子（NO₃⁻）或氧气（O₂）可恢复该突变体中的DNA生物合成。厌氧状态对该突变体中放射性腺嘌呤的进入没有明显影响。由此得出结论，偶联因子在DNA生物合成的某些步骤中起作用。
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