抗人脑源性神经营养因子单克隆抗体的制备与鉴定
Preparation and identification of monoclonal antibodies against human brain-derived neurotrophic factor.
作者信息
Su Jin, Zhang Ya-Li, You Chang-Xuan, Wu Jin-Ya, Che Xiao-Yan, Wang Xiao-Ning
机构信息
Department of Respiratory Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2002 Oct;22(10):944-5.
OBJECTIVE
To prepare monoclonal antibodies (mAbs) against human brain-derived neurotrophic factor (BDNF).
METHODS
BALB/C mice were immunized with purified BDNF protein in conjunction with acid-treated Salmonella (antigen:thallus=1:5), and mAbs were subsequently derived by hybridoma technique.
RESULTS
Three hybridoma cell lines secreting anti-BDNF mAbs were obtained, designated as B1, B2 and 4D1 respectively and all categorized into IgG1 subtype. The titers of the mAbs in the ascitic fluid of the rats ranged from 1x10(6) to 1x10(5), and their relative affinities were B2>B1>4D1.
CONCLUSION
mAbs against BDNF are successfully prepared, which can be instrumental for further study of the expression and distribution of BDNF in vivo, and the detection of BDNF produced by genetic engineering.
目的
制备抗人脑源性神经营养因子（BDNF）的单克隆抗体（mAb）。
方法
用纯化的BDNF蛋白与经酸处理的沙门氏菌（抗原：菌体 = 1:5）联合免疫BALB/C小鼠，随后通过杂交瘤技术获得单克隆抗体。
结果
获得了三株分泌抗BDNF单克隆抗体的杂交瘤细胞系，分别命名为B1、B2和4D1，均归类为IgG1亚型。大鼠腹水中单克隆抗体的效价范围为1×10⁶至1×10⁵，其相对亲和力为B2 > B1 > 4D1。
结论
成功制备了抗BDNF的单克隆抗体，这有助于进一步研究BDNF在体内的表达和分布，以及检测基因工程产生的BDNF。
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