Mahlamäki Eija H, Bärlund Maarit, Tanner Minna, Gorunova Ludmila, Höglund Mattias, Karhu Ritva, Kallioniemi Anne
Laboratory of Cancer Genetics, Tampere University Hospital and University of Tampere, Tampere, Finland.
Genes Chromosomes Cancer. 2002 Dec;35(4):353-8. doi: 10.1002/gcc.10122.
Genetic changes involved in the development and progression of pancreatic cancer are still partly unknown, despite the progress in recent years. In this study, comparative genomic hybridization analysis in 31 pancreatic cancer cell lines showed that chromosome arms 8q, 11q, 17q, and 20q are frequently gained in this tumor type. Copy number analysis of selected genes from these chromosome arms by fluorescence in situ hybridization showed amplification of the MYC oncogene in 54% of the cell lines, whereas CCND1 was amplified in 28%. In the 17q arm, the ERBB2 oncogene was amplified in 20% of the cell lines, TBX2 in 50%, and BIRC5 in 58%, indicating increased involvement toward the q telomere of chromosome 17. In the 20q arm, the amplification frequencies varied from 32% to 83%, with the CTSZ gene at 20q13 being most frequently affected. These results illustrate that amplification of genes from the 8q, 11q, 17q, and 20q chromosome arms is common in pancreatic cancer.
尽管近年来取得了进展，但胰腺癌发生和发展过程中涉及的基因变化仍部分未知。在本研究中，对31个胰腺癌细胞系进行的比较基因组杂交分析表明，8q、11q、17q和20q染色体臂在这种肿瘤类型中经常出现扩增。通过荧光原位杂交对这些染色体臂上选定基因的拷贝数分析显示，54%的细胞系中MYC癌基因发生扩增，而CCND1扩增的细胞系占28%。在17q染色体臂上，20%的细胞系中ERBB2癌基因扩增，50%的细胞系中TBX2扩增，58%的细胞系中BIRC5扩增，表明向17号染色体q端粒的参与增加。在20q染色体臂上，扩增频率从32%到83%不等，其中位于20q13的CTSZ基因受影响最为频繁。这些结果表明，8q、11q、17q和20q染色体臂上的基因扩增在胰腺癌中很常见。
