Differences in ratios of GABA, glycine and glutamate immunoreactivities in nerve terminals on rat hindlimb motoneurons: a possible source of post-synaptic variability.

Previous pharmacological and physiological data on GABA and glycine receptor-dependent components of miniature inhibitory post-synaptic currents show that the electrophysiological characteristics of synaptic transmission from inhibitory synapses on spinal motoneurons are highly variable. Although post-synaptic factors are thought to be the major underlying cause of this variability, quantitative immunohistochemical data suggest that the transmitter content of afferents also vary from terminal to terminal. To examine whether ratios of amino acid staining densities vary similar to those of components of post-synaptic currents mediated by the corresponding receptors, we quantified immunogold labeling for GABA, glycine and the major excitatory transmitter, glutamate, in nerve terminals contacting the dendrites of motoneurons retrogradely labeled from the rat hindlimb muscle, biceps femoris. Nearly all terminals (94%) were immunoreactive for at least one amino acid and 64% of these contained two or three amino acids. All possible combinations of GABA, glycine and glutamate labeling were found. Over 70% of the terminals contained glycine, of which 60% also labeled for GABA. Of these GABA/glycine boutons, 40% also had glutamate. Half of all terminals contained GABA, but terminals immunoreactive for GABA alone were extremely rare. Immunoreactivity for glutamate occurred in 48% of all terminals and nearly 60% of these also contained glycine. Labeling densities for GABA, glycine and glutamate varied over a wide range from terminal to terminal. We hypothesize that this diversity in amino acid content may be a major underlying cause of variability in GABA- and glycine receptor-mediated components of miniature inhibitory post-synaptic currents in motoneurons.