Vial J, Porter K R
J Cell Biol. 1975 Nov;67(2PT.1):345-60. doi: 10.1083/jcb.67.2.345.
A method is described for studying by scanning electron microscopy (SEM) all the surfaces of fully differentiated cells from intact tissues. Thus, cell faces normally hidden from view are exposed and made available for SEM examination. This is achieved by fixing the tissue in OSO4 and then soaking it in a 1% solution (in water) of boric acid. After different periods of time, varied according to particular tissue, slight mechanical pressure will cause the fixed tissue to dissociate into its component cells. These are then made to adhere to a substrate and are taken through critical point drying, etc., for examination. Observations are reported on the topography of whole hepatocytes, adsorptive cells of the intestinal epithelium, proximal tubule cells of the rat kidney, mammary tumor cells of the mouse, and rat sarcoma cells. Several other tissues are reported to dissociate when similarly treated, but for each the procedure must be slightly modified.
本文描述了一种通过扫描电子显微镜（SEM）研究完整组织中完全分化细胞所有表面的方法。这样，通常隐藏不可见的细胞面得以暴露并可用于SEM检查。这是通过将组织固定在四氧化锇中，然后将其浸泡在1%（水溶液）的硼酸溶液中来实现的。经过根据特定组织而变化的不同时间段后，轻微的机械压力会使固定的组织解离成其组成细胞。然后使这些细胞附着在基质上，并经过临界点干燥等处理以进行检查。报告了对整个肝细胞、肠上皮吸附细胞、大鼠肾近端小管细胞、小鼠乳腺肿瘤细胞和大鼠肉瘤细胞的表面形貌观察结果。据报道，其他几种组织在经过类似处理时也会解离，但对于每种组织，该程序必须稍作修改。
