90%肝切除术后经腹腔移植的大鼠肝细胞微囊化多细胞球体。

Microencapsulated multicellular spheroid of rat hepatocytes transplanted intraperitoneally after 90% hepatectomy.

作者信息

Hamazaki Keisuke, Doi Yosinori, Koide Norio

机构信息

First Department of Surgery, Okayama University Medical School, Shikata-cho 2-5-1, Okayama City, 700-8558, Japan.

出版信息

Hepatogastroenterology. 2002 Nov-Dec;49(48):1514-6.

PMID:12397721

Abstract

BACKGROUND/AIMS: The aim of this preliminary study was to investigate the metabolic activity of hepatocyte spheroids encapsulated with agarosehydrogel in vitro and the effect of encapsulated hepatocyte spheroids transplanted intraperitoneally on survival in the 90% hepatectomized rats for preparing future xeno-hepatocyte spheroids transplantation.

METHODOLOGY

Hepatocytes were prepared by Seglen's method and cultured with epidermal growth factor. Hepatocytes formed spheroids 4 days after inoculation. Hepatocyte spheroids were encapsulated into 5% concentration agarose-hydrogel microbeads before being transplanted into the peritoneum of the rats.

RESULTS

Hepatocyte spherois showed better metabolic activity compared to isolated hepatocytes. Hepatocyte spheroids function cultured in vitro were not deteriorated by encapsulation with agarose-hydrogel. Survival rates of 90% hepatectomized rats were improved by the intraperitoneal transplantation of encapsulated hepatocyte spheroids.

CONCLUSIONS

Rat hepatocyte spheroids encapsulated with agarose-hydrogel and transplanted intraperitoneally were considered as effective for the improvement of survival rates in acute liver failure produced by 90% hepatectomy.

摘要

背景/目的：本初步研究旨在体外研究包裹于琼脂糖水凝胶中的肝细胞球的代谢活性，以及腹腔内移植包裹的肝细胞球对90%肝切除大鼠存活率的影响，以便为未来的异种肝细胞球移植做准备。