Wanga J, Duttaroy A, Anderson W A
Department of Biology, Howard University, Washington, DC 20059, USA.
J Submicrosc Cytol Pathol. 2002 Jul;34(3):233-9.
Expression of NK2 homologous transcripts during Zebrafish development was investigated by in situ hybridization technique. NK2 mRNA expression was first detected in a cluster of cells at the embryonic shield stage, and subsequently in the heart field of the 5-8-somite embryo. By the 15-17 somite stages, NK2 expressing cells were revealed in the midline of the developing embryo, and by 48 hpf within the heart tube of the larva. By 72 hpf, NK2 transcripts appeared in the jaw bones, pharyngeal arches, the cranium with minimal expression in striated muscle.
采用原位杂交技术研究了斑马鱼发育过程中NK2同源转录本的表达情况。NK2 mRNA表达首先在胚胎盾期的一群细胞中被检测到,随后在5-8体节胚胎的心脏区域被检测到。到15-17体节期,在发育中胚胎的中线处发现了表达NK2的细胞,到48小时胚胎期时,在幼虫的心脏管内也有表达。到72小时胚胎期时,NK2转录本出现在颌骨、咽弓、颅骨中,在横纹肌中的表达极少。