Granert Carl, Raud Johan, Lindquist Lars, Lindbom Lennart
Department of Infectious Diseases, Karolinska Institute, I 73, Huddinge University Hospital, Sweden.
Med Microbiol Immunol. 2002 Oct;191(2):97-100. doi: 10.1007/s00430-002-0114-5. Epub 2002 Apr 3.
Inflammatory recruitment of leukocytes into the cerebrospinal fluid (CSF) during bacterial meningitis has been shown to contribute to the neurological damage commonly associated with this disease. In this study we tested whether inhibition of firm leukocyte adhesion to vascular endothelium could reduce leukocyte recruitment into the subarachnoid space (SAS) and into the skin in rabbits challenged with pneumococcal cell wall (PCW) antigen. PCW was given either as an intracisternal or an intradermal (i.d.) injection. Intravenous (i.v.) treatment with a monoclonal antibody (mAb), IB4, against the leukocytic adhesion molecule CD18 has previously been documented to attenuate leukocyte CSF accumulation in experimental bacterial meningitis. In the present study, i.v. treatment with anti-CD18 mAbs (IB4) only tended to inhibit CSF leukocyte influx in animals with PCW-induced meningitis. However, if the antigen was injected i.d., treatment i.v. with the same mAb (IB4) dramatically reduced leukocyte accumulation in the skin. Our findings indicate that the mechanisms responsible for PCW-induced inflammatory accumulation of leukocytes in skin and meninges are different.
细菌性脑膜炎期间，白细胞向脑脊液（CSF）的炎症性募集已被证明会导致与该疾病通常相关的神经损伤。在本研究中，我们测试了抑制白细胞与血管内皮的牢固黏附是否能减少肺炎球菌细胞壁（PCW）抗原攻击的家兔白细胞向蛛网膜下腔（SAS）和皮肤的募集。PCW通过脑池内或皮内（i.d.）注射给予。先前有文献记载，静脉内（i.v.）用抗白细胞黏附分子CD18的单克隆抗体（mAb）IB4治疗可减轻实验性细菌性脑膜炎中白细胞在脑脊液中的积聚。在本研究中，静脉内用抗CD18单克隆抗体（IB4）治疗仅倾向于抑制PCW诱导的脑膜炎动物脑脊液中白细胞的流入。然而，如果抗原通过皮内注射，静脉内用相同的单克隆抗体（IB4）治疗可显著减少皮肤中白细胞的积聚。我们的研究结果表明，PCW诱导白细胞在皮肤和脑膜中炎症性积聚的机制是不同的。
