一种来自新型pVII-pIX噬菌体展示随机肽库的细胞穿透肽。
A cell-penetrating peptide from a novel pVII-pIX phage-displayed random peptide library.
作者信息
Gao Changshou, Mao Shenlan, Ditzel Henrik J, Farnaes Lauge, Wirsching Peter, Lerner Richard A, Janda Kim D
机构信息
Department of Chemistry, The Scripps Research Institute and the Skaggs Institute for Chemical Biology, La Jolla, CA 92037, USA.
出版信息
Bioorg Med Chem. 2002 Dec;10(12):4057-65. doi: 10.1016/s0968-0896(02)00340-1.
A novel random peptide library was constructed using a phage-display format on the coat proteins pVII and pIX of filamentous bacteriophage. Panning against B-lymphocyte WI-L2 cells yielded one unique peptide-phage, denoted CHL8, that specifically bound to and penetrated the cells. Studies of each peptide derived from CHL8, denoted pep7 and pep9, established that only pep7 mediated the observed activity and only as a homodimer. Peptide libraries displayed on pVII-pIX should serve as a novel source of bioactive ligands for a variety of applications.
利用丝状噬菌体外壳蛋白pVII和pIX上的噬菌体展示形式构建了一个新型随机肽库。针对B淋巴细胞WI-L2细胞进行淘选，得到一种独特的肽噬菌体，命名为CHL8，它能特异性结合并穿透细胞。对源自CHL8的每种肽（分别命名为pep7和pep9）的研究表明，只有pep7介导了观察到的活性，且仅作为同二聚体发挥作用。展示在pVII - pIX上的肽库应可作为用于多种应用的生物活性配体的新型来源。
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