Zhao Kong-Nan, Frazer Ian H
Centre for Immunology and Cancer Research, The University of Queensland, Princess Alexandra Hospital, Brisbane, Queensland 4102, Australia.
J Virol. 2002 Dec;76(23):12265-73. doi: 10.1128/jvi.76.23.12265-12273.2002.
We recently demonstrated that Saccharomyces cerevisiae protoplasts can take up bovine papillomavirus type 1 (BPV1) virions and that viral episomal DNA is replicated after uptake. Here we demonstrate that BPV virus-like particles are assembled in infected S. cerevisiae cultures from newly synthesized capsid proteins and also package newly synthesized DNA, including full-length and truncated viral DNA and S. cerevisiae-derived DNA. Virus particles prepared in S. cerevisiae are able to convey packaged DNA to Cos1 cells and to transform C127 cells. Infectivity was blocked by antisera to BPV1 L1 but not antisera to BPV1 E4. We conclude that S. cerevisiae is permissive for the replication of BPV1 virus.
我们最近证明，酿酒酵母原生质体能摄取1型牛乳头瘤病毒（BPV1）病毒粒子，并且摄取后病毒游离型DNA会复制。在此我们证明，BPV病毒样颗粒在受感染的酿酒酵母培养物中由新合成的衣壳蛋白组装而成，并且还包装新合成的DNA，包括全长和截短的病毒DNA以及酿酒酵母来源的DNA。在酿酒酵母中制备的病毒粒子能够将包装的DNA传递给Cos1细胞并转化C127细胞。抗BPV1 L1血清可阻断感染性，但抗BPV1 E4血清则不能。我们得出结论，酿酒酵母允许BPV1病毒复制。
