[转化生长因子-β1和Smad 2 mRNA在病变肾小球中的表达及其在肾小球硬化发生发展中的意义]
[The expressions of TGF-beta1 and Smad 2 mRNA on diseased glomeruli and their significance in the development of glomerulosclerosis].
作者信息
Ruan Yonghua, Zhang Zhigang, Zhang Xiurong, Liu Chen, Guo Muyi
机构信息
Department of Pathology, Medical Center of Fudan University, Shanghai 200032, China.
出版信息
Zhonghua Bing Li Xue Za Zhi. 2002 Aug;31(4):314-7.
OBJECTIVE
To explore the expressions of transforming growth factor-beta1 (TGF-beta1) and its signaling transduction molecule Smad 2 and their significance in the development of glomerulosclerosis.
METHODS
Using in situ hybridization and immunohistochemistry to detect Smad 2 mRNA expression and TGF-beta1, collagen IV, fibronectin expression in renal biopsies from 61 cases with a spectrum of glomerulonephritis including IgA nephropathy (40 cases), membranous glomerulonephritis (10 cases) and sclerosing glomerulonephritis (11 cases), compared with 11 cases of glomerular mild lesion with image analysis system.
RESULTS
With the exception of Smad 2 mRNA expression in mild type IgA nephropathy, all other types of diseased glomeruli showed increased expression of both TGF-beta1 and Smad 2 mRNA when compared with the 11 cases of mild glomerular lesions. The expressions of glomerular TGF-beta1 and Smad 2 mRNA positively correlated with collagen IV and fibronectin deposition in the glomeruli.
CONCLUSIONS
TGF-beta1 and Smad 2 may be involved in the excessive deposition of glomerular extracellular matrix and play an important role in the development of glomerulosclerosis.
目的
探讨转化生长因子-β1（TGF-β1）及其信号转导分子Smad 2在肾小球硬化发生发展中的表达及其意义。
方法
采用原位杂交和免疫组化方法检测61例不同类型肾小球肾炎患者肾活检组织中Smad 2 mRNA表达及TGF-β1、IV型胶原、纤维连接蛋白的表达，其中包括IgA肾病40例、膜性肾小球肾炎10例、硬化性肾小球肾炎11例，并与11例肾小球轻度病变患者作对照，用图像分析系统进行分析。
结果
除轻度系膜增生性IgA肾病患者Smad 2 mRNA表达外，其他各型病变肾小球TGF-β1和Smad 2 mRNA表达均高于11例肾小球轻度病变患者。肾小球TGF-β1和Smad 2 mRNA表达与肾小球IV型胶原和纤维连接蛋白沉积呈正相关。
结论
TGF-β1和Smad 2可能参与肾小球细胞外基质的过度沉积，在肾小球硬化发生发展中起重要作用。
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