Segurado Mónica, Gómez María, Antequera Francisco
Instituto de Microbiología Bioquímica, CSIC/Universidad de Salamanca, Edificio Departamental, Campus Miguel de Unamuno, 37007, Salamanca, Spain.
Mol Cell. 2002 Oct;10(4):907-16. doi: 10.1016/s1097-2765(02)00684-6.
We have studied the relationship between DNA replication and recombination in Schizosaccharomyces pombe using two-dimensional gel electrophoresis and functional analysis. Our results indicate that the activation of replication origins (ORIs) during the mitotic cell cycle is associated with the generation of joint DNA molecules between sister chromatids. The frequency of integration by homologous recombination was up to 50-fold higher than the genomic average within a narrow window overlapping the ars1 replication initiation site. The S. pombe rad22Delta, rhp51Delta, and rhp54Delta mutants, deficient in mitotic recombination, activate ORIs very inefficiently and accumulate abnormal replication intermediates. These results focus on the general link between replication and recombination previously found in several systems and suggest a role for recombination in the initiation of eukaryotic DNA replication.
我们利用二维凝胶电泳和功能分析研究了粟酒裂殖酵母中DNA复制与重组之间的关系。我们的结果表明，有丝分裂细胞周期中复制起点（ORIs）的激活与姐妹染色单体之间的联合DNA分子的产生有关。在与ars1复制起始位点重叠的狭窄窗口内，同源重组的整合频率比基因组平均水平高50倍。缺乏有丝分裂重组的粟酒裂殖酵母rad22Delta、rhp51Delta和rhp54Delta突变体激活ORIs的效率非常低，并积累异常的复制中间体。这些结果聚焦于先前在几个系统中发现的复制与重组之间的普遍联系，并暗示重组在真核生物DNA复制起始中发挥作用。
