Lane Michelle, Maybach Jeffrey M, Hooper Kathy, Hasler John F, Gardner David K
Research Department, Colorado Center for Reproductive Medicine, Englewood, Colorado 80110, USA.
Mol Reprod Dev. 2003 Jan;64(1):70-8. doi: 10.1002/mrd.10210.
Recombinant albumin can be used to supplement culture medium for the maturation and fertilization of bovine oocytes and subsequent embryo development to the blastocyst stage. Recombinant albumin was able to support blastocyst development at rates equivalent to that of bovine serum albumin (BSA) supplemented media. Supplementation of media containing recombinant albumin and citrate stimulated blastocyst expansion. Culture with recombinant albumin and citrate significantly increased the ability of the resultant blastocysts to re-expand and hatch following cryopreservation. The further addition of the glycosaminoglycan hyaluronan to the culture medium containing either BSA or recombinant albumin also increased the ability of blastocysts to survive cryopreservation. Inclusion of recombinant albumin and hyaluronan in culture media facilitates the development of physiological defined culture conditions. For bovine embryos this has implications for both research and commercial applications where defined reproducible conditions are desirable.
重组白蛋白可用于补充培养基，以促进牛卵母细胞的成熟和受精，并使随后的胚胎发育至囊胚阶段。重组白蛋白能够以与补充牛血清白蛋白（BSA）的培养基相当的速率支持囊胚发育。含有重组白蛋白和柠檬酸盐的培养基的补充刺激了囊胚扩张。用重组白蛋白和柠檬酸盐培养显著提高了所得囊胚在冷冻保存后重新扩张和孵化的能力。向含有BSA或重组白蛋白的培养基中进一步添加糖胺聚糖透明质酸也提高了囊胚在冷冻保存后的存活能力。在培养基中加入重组白蛋白和透明质酸有助于建立生理明确的培养条件。对于牛胚胎来说，这对需要明确可重复条件的研究和商业应用都有影响。
Zotero 插件