Raue R, Hafez H M, Hess M
Institute of Poultry Diseases, Free University Berlin, Koserstrasse 21, Germany.
Avian Pathol. 2002 Feb;31(1):95-9. doi: 10.1080/03079450120106660.
A polymerase chain reaction (PCR) was developed to detect a recently described pigeon adenovirus (PiAV). Primers located in the fiber gene of PiAV amplified a PCR fragment solely from PiAV DNA, whereas all 12 serotypes of fowl adenoviruses (FAV1 to FAV12), some of them able to infect pigeons, did not react. A PCR fragment of 967 base pairs was amplified from three different isolates serologically typed as PiAV and from some pigeon liver samples showing morphological and histological signs of an adenovirus infection. Those samples did not react with a published primer pair (H3/H4) able to detect FAV, demonstrating the specificity of both PCRs to react exclusively with the respective pathogen, PiAV or FAV. The presented PCR is a suitable diagnostic tool to gain further insight into the epidemiology of PiAV infections in pigeons.
已开发出一种聚合酶链反应（PCR）来检测最近描述的鸽腺病毒（PiAV）。位于PiAV纤维基因中的引物仅从PiAV DNA中扩增出一个PCR片段，而12种禽腺病毒血清型（FAV1至FAV12），其中一些能够感染鸽子，却无反应。从三种血清学鉴定为PiAV的不同分离株以及一些显示腺病毒感染形态学和组织学迹象的鸽肝样本中扩增出了一个967个碱基对的PCR片段。这些样本与能够检测FAV的已发表引物对（H3/H4）无反应，这证明了两种PCR分别仅与各自病原体PiAV或FAV反应的特异性。所呈现的PCR是一种合适的诊断工具，有助于进一步了解鸽PiAV感染的流行病学情况。
