以皮肤成纤维细胞为种子细胞的组织工程化肌腱：初步研究

[Tissue engineered tendon with skin fibroblasts as seed cells, a preliminary study].

作者信息

Chen Bing, Ding Xiaobang, Liu Fangjun, Cui Lei, Liu Wei, Shang Qingxin, Cao Yilin

机构信息

The Research Center of Tissue Engineering, Department of Plastic Surgery, Ninth People's Hospital Affiliated to Shanghai Second Medical University, Shanghai 200011, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2002 Aug 25;82(16):1105-7.

PMID:12425819

Abstract

OBJECTIVE

To investigate the effect of tissue engineered tendon using autologous dermal fibroblasts in repairing tendon damage.

METHODS

Skin tissues were resected from abdomen of 5 pigs. Fibroblasts were isolated from the skin pieces and cultured in vitro. Bundles of polyglycolic acid (PGA) were arranged in parallel and mixed with the suspension of fibroblasts to form a cell-scaffolded construct, which was further cultured in vitro for 1 week. The tendon of musculus flexor digitorum superficialis of pig's right leg was cut with a defect 3 cm long and then bridged with the cultured construct. Six weeks later, specimens of the regenerated tendon of right musculus flexor digitorum superficialis and specimens of corresponding left leg were taken for gross examination, microscopy and biomechanical analysis.

RESULTS

After implantation of the fibroblast-biomaterial complex the wounds healed up and the pigs moved well. The histology of the implanted tendon was similar to that of natural tendon. The breaking strength and maximum tensile force were 173.0 +/- 18.2 N and 18.9 +/- 1.9 MPa, reaching 57.4% and 51.9% of those of normal tendon respectively.

CONCLUSION

Skin fibroblasts can be used as seed cells to regenerate tendon with normal structure and function.

摘要

目的

研究采用自体真皮成纤维细胞构建的组织工程化肌腱修复肌腱损伤的效果。

方法

从5头猪的腹部切取皮肤组织。从皮肤块中分离出成纤维细胞并进行体外培养。将聚乙醇酸（PGA）束平行排列并与成纤维细胞悬液混合，形成细胞-支架构建体，再进一步体外培养1周。切断猪右下肢指浅屈肌肌腱，造成3 cm长的缺损，然后用培养好的构建体进行桥接修复。6周后，取右下肢指浅屈肌再生肌腱标本及相应左下肢标本进行大体观察、显微镜检查和生物力学分析。