传染性法氏囊病病毒在不同宿主系统（法氏囊、胚胎和细胞培养）中增殖所导致的抗原性、免疫原性和致病性变异的特征。I. 抗原性和免疫原性。
Characterization of the antigenic, immunogenic, and pathogenic variation of infectious bursal disease virus due to propagation in different host systems (bursa, embryo, and cell culture). I. Antigenicity and immunogenicity.
作者信息
Rodriguez-Chavez Isaac R, Rosenberger John K, Cloud Sandra S
机构信息
Department of Animal and Food Sciences, Allen Biotechnology Laboratory, University of Delaware, 601 Sincock Lane, Newark, DE 19717, USA.
出版信息
Avian Pathol. 2002 Oct;31(5):463-71. doi: 10.1080/0307945021000005833.
In vitro and in ovo virus neutralization assays were conducted to assess the role of different host systems in infectious bursal disease virus (IBDV) antigenic and immunogenic variation. Four different strains, two variant (1084 E and GLS) and two standard (Edgar and STC), were propagated separately in the bursa of Fabricius and embryos, and were compared with cell culture-adapted preparations of the homologous strains. Chicken polyclonal antisera were prepared against each IBDV and neutralizing antibody titres were determined. Normalized IBDV antibody concentrations were used in neutralization assays against homologous and heterologous IBDVs in 10-day-old specific pathogen free embryos. Both antigenic and immunogenic changes occurred in IBDVs evaluated, as evidenced by differences in the ability of normalized antibody to neutralize IBDV propagated in different host systems. Antibody induced by bursal-derived IBDV neutralized all isolates equally well, whereas antibody induced by cell culture-derived virus neutralized bursal-derived IBDV much less effectively.
进行了体外和卵内病毒中和试验，以评估不同宿主系统在传染性法氏囊病病毒（IBDV）抗原性和免疫原性变异中的作用。四种不同的毒株，两种变异株（1084 E和GLS）和两种标准株（Edgar和STC），分别在法氏囊和胚胎中繁殖，并与同源毒株的细胞培养适应制剂进行比较。制备了针对每种IBDV的鸡多克隆抗血清，并测定了中和抗体滴度。标准化的IBDV抗体浓度用于针对10日龄无特定病原体胚胎中的同源和异源IBDV进行中和试验。在所评估的IBDV中发生了抗原性和免疫原性变化，标准化抗体中和在不同宿主系统中繁殖的IBDV的能力差异证明了这一点。法氏囊来源的IBDV诱导的抗体对所有分离株的中和效果相同，而细胞培养来源的病毒诱导的抗体对法氏囊来源的IBDV的中和效果则差得多。
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