纤维蛋白D - 二聚体片段中的一种新抗原决定簇。
A neoantigenic determinant in the D-dimer fragment of fibrin.
作者信息
Lugovskoy E V, Kolesnikova I N, Gritsenko P G, Zolotareva E N, Gaffney P, Nieuwenhuizen W, Komisarenko S V
机构信息
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, 9 Leontovicha street, Kiev 01601, Ukraine.
出版信息
Thromb Res. 2002 Aug 15;107(3-4):151-6. doi: 10.1016/s0049-3848(02)00204-9.
Monoclonal antibody (mAb) III-3b binds D-dimer with K(d)=1.4 x 10(-10) M without cross-reaction with fibrin(ogen). The epitope for this mAb is in Bbeta134-190, presumably in Bbeta155-160. The latter site is buried in the coiled coil structure of fibrin(ogen) but it is exposed as a neoantigenic determinant in D-dimer upon plasmic lysis of fibrin. mAb III-3b may be used as a tool for immunodiagnostic quantification of D-dimer in blood plasma.
单克隆抗体（mAb）III-3b与D-二聚体结合，解离常数K(d)=1.4×10⁻¹⁰ M，且与纤维蛋白（原）无交叉反应。该单克隆抗体的表位位于Bβ134 - 190，推测在Bβ155 - 160。后一位点埋藏于纤维蛋白（原）的卷曲螺旋结构中，但在纤维蛋白发生血浆溶解时，它作为D-二聚体中的新抗原决定簇暴露出来。单克隆抗体III-3b可用作免疫诊断定量检测血浆中D-二聚体的工具。
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