Reuss S, Saaler-Reinhardt S, Weich B, Wystub S, Reuss M H, Burmester T, Hankeln T
Department of Anatomy, Johannes Gutenberg University, 55099, Mainz, Germany.
Neuroscience. 2002;115(3):645-56. doi: 10.1016/s0306-4522(02)00536-5.
Neuroglobin is a respiratory protein which was reported to be preferentially expressed in the vertebrate brain. Here we present the first detailed analysis of the expression of neuroglobin in mouse and rat tissues. Neuroglobin mRNA was detected in all brain areas studied. Most, but not all, nerve cells were labeled, suggesting differential expression of Ngb. Neuroglobin mRNA was detected in the peripheral nervous system, explaining previous northern hybridization signals in organs other than the brain. Substantial neuroglobin expression was also found in metabolically active endocrine tissues such as the adrenal and pituitary glands. The granule localization of neuroglobin transcripts in various neuronal extensions let us speculate that peripheral translation of neuroglobin protein occurs. This could have important functional consequences for synaptic plasticity, an active metabolic process that needs large amounts of oxygen. The hybridization signals suggest that the local concentration of neuroglobin is sufficient for its putative primary function as an oxygen-supplying protein.
神经球蛋白是一种呼吸蛋白，据报道它在脊椎动物大脑中优先表达。在此，我们首次对神经球蛋白在小鼠和大鼠组织中的表达进行了详细分析。在所研究的所有脑区均检测到神经球蛋白mRNA。大多数（但并非全部）神经细胞被标记，这表明神经球蛋白存在差异表达。在周围神经系统中检测到神经球蛋白mRNA，这解释了之前在脑以外器官中的Northern杂交信号。在代谢活跃的内分泌组织如肾上腺和垂体中也发现了大量的神经球蛋白表达。神经球蛋白转录本在各种神经元突起中的颗粒定位使我们推测神经球蛋白蛋白存在外周翻译。这可能对突触可塑性产生重要的功能影响，突触可塑性是一个需要大量氧气的活跃代谢过程。杂交信号表明神经球蛋白的局部浓度足以实现其作为供氧蛋白的假定主要功能。
