[未分化鼻咽癌中Fas/Fas配体表达与细胞凋亡的相关性]

[Correlation between Fas/Fas-ligand expression and apoptosis in undifferentiated nasopharyngeal carcinoma].

作者信息

Enami Atsuto, Tsutsumi Kouiciro, Kobayashi Takehiko, Shibosawa Eriko, Koizuka Izumi

机构信息

Department of Otolaryngology, St. Marianna University School of Medicine, Kanagawa.

出版信息

Nihon Jibiinkoka Gakkai Kaiho. 2002 Oct;105(10):1087-92. doi: 10.3950/jibiinkoka.105.1087.

DOI:10.3950/jibiinkoka.105.1087

PMID:12440162

Abstract

We determined whether resistance to Fas-mediated apoptosis is involved in the development of undifferentiated nasopharyngeal carcinomas (UNPCs). In UNPC tissue sections from 20 cases, we conducted immunohistochemistry to detect Fas and FasL, in situ hybridization to detect Epstein-Barr virus-encoded small RNA (EBER), and immunohistochemistry for single-stranded DNA (ssDNA) to detect apoptotic cells. Fas was detected in 17/20 (85%), FasL in 15/20 (75%), and EBER in 16/20 (80%). Fas was detected in all 16 EBER-positive UNPCs, but not in 3 (75%) of 4 EBER-negative UNPCs. FasL was detected in 12 (75%) of 16 EBER-positive UNPCs and 3 (75%) of 4 EBER-negative UNPCs. Interestingly, despite Fas and FasL co-expression in UNPC cells (Fas+/FasL+ cases = 13/20 or 65%), apoptotic UNPC cells were significantly lower in Fas+/FasL+ than in other cases. These results suggest that Fas+/FasL+ UNPC cells are resistant to Fas-mediated apoptosis in vivo by mechanisms excluding lack of Fas expression.

摘要

我们确定了对Fas介导的细胞凋亡的抗性是否参与未分化鼻咽癌（UNPC）的发生发展。在20例UNPC组织切片中，我们进行了免疫组织化学检测Fas和FasL，原位杂交检测爱泼斯坦-巴尔病毒编码的小RNA（EBER），以及单链DNA（ssDNA）免疫组织化学检测凋亡细胞。在20例中的17例（85%）检测到Fas，20例中的15例（75%）检测到FasL，20例中的16例（80%）检测到EBER。在所有16例EBER阳性的UNPC中均检测到Fas，但在4例EBER阴性的UNPC中的3例（75%）未检测到。在16例EBER阳性的UNPC中的12例（75%）和4例EBER阴性的UNPC中的3例（75%）检测到FasL。有趣的是，尽管UNPC细胞中Fas和FasL共表达（Fas+/FasL+病例 = 13/20或65%），但Fas+/FasL+的凋亡UNPC细胞明显低于其他病例。这些结果表明，Fas+/FasL+的UNPC细胞在体内对Fas介导的细胞凋亡具有抗性，其机制不包括缺乏Fas表达。