Schwartz O, Andreasen F M, Andreasen J O
Department of Oral and Maxillofacial Surgery, University Hospital Rigshospitalet Copenhagen, Denmark.
Dent Traumatol. 2002 Aug;18(4):190-5. doi: 10.1034/j.1600-9657.2002.00086.x.
The effect of temperature of various storage media and at varying storage periods upon periodontal ligament (PDL) and pulpal healing after tooth replantation was examined in green vervet monkeys (Cercopithecus aethiops). Mandibular lateral incisors with mature root formation were extracted and kept in dry storage at 22, 4 and -18 degrees C; in saline at 37, 22, 4 and -18 degrees C; or in saliva (i.e. in the buccal vestibule) at 37 degrees C for either 60 or 120 min prior to replantation. The animals were sacrificed 8 weeks after replantation and the replanted teeth examined histometrically. The following histological parameters were registered for each tooth: normal PDL, surface resorption, inflammatory resorption, replacement resorption (ankylosis), downgrowth of pocket epithelium, periapical inflammatory changes, and the extent of vital pulp. A total of 125 replanted teeth were examined. Storage in saliva at 37 degrees C showed a similar amount of normal PDL compared to saline storage for both 60 and 120 min. Saline storage for 60 or 120 min showed no difference in the extent of normal PDL when storage was compared at 37, 22 and 4 degrees C. However, storage at -18 degrees C resulted in significantly less normal PDL than storage at other temperatures. Dry storage for 60 min showed significantly less root resorption at 4 degrees C compared to 22 degrees C. Furthermore, dry storage at -18 degrees C showed significantly less normal PDL than storage at 4 degrees C. When the dry storage period was extended to 120 min, no difference was found between 22, 4 and -18 degrees C. It is concluded that the temperature (above 0 degrees C) of the storage medium is of importance only for dry storage and in such a situation only for shorter extra-alveolar periods, i.e. for 60-min storage and not for 120 min, where extensive destruction of the PDL always takes place. It is suggested that the temperature effect of 4 degrees C could be related to less evaporation from the PDL and thereby less damage to the PDL cells or a strict temperature effect upon cell metabolism. Pulp healing in all the cases was limited to the entrance of the pulp canal, and no significant pattern was found between storage media, time and temperature.
在绿长尾猴（猕猴属埃塞俄比亚种）中，研究了不同储存介质的温度以及不同储存时长对牙再植后牙周膜（PDL）和牙髓愈合的影响。提取牙根发育成熟的下颌侧切牙，分别在22℃、4℃和-18℃下干储存；在37℃、22℃、4℃和-18℃的盐水中储存；或在37℃的唾液中（即口腔前庭）储存60或120分钟，然后再进行牙再植。牙再植8周后处死动物，对再植牙进行组织计量学检查。记录每颗牙的以下组织学参数：正常牙周膜、表面吸收、炎性吸收、替代性吸收（粘连）、牙周袋上皮向下生长、根尖炎性改变以及活髓范围。共检查了125颗再植牙。与在盐水中储存60和120分钟相比，在37℃唾液中储存的正常牙周膜量相似。当在37℃、22℃和4℃下比较储存时，在盐水中储存60或120分钟的正常牙周膜范围无差异。然而，与在其他温度下储存相比，在-18℃下储存导致正常牙周膜明显减少。与22℃相比，在4℃下干储存60分钟显示牙根吸收明显减少。此外，与在4℃下储存相比，在-18℃下干储存显示正常牙周膜明显减少。当干储存时长延长至120分钟时，在22℃、4℃和-18℃之间未发现差异。得出结论，储存介质的温度（高于0℃）仅对干储存重要，且仅在这种情况下对较短的牙槽外时长重要，即对于60分钟的储存而非120分钟的储存，因为120分钟时牙周膜总会发生广泛破坏。有人提出4℃的温度效应可能与牙周膜水分蒸发减少从而对牙周膜细胞损伤较小有关，或者与对细胞代谢的严格温度效应有关。在所有情况下，牙髓愈合均局限于牙髓腔入口，在储存介质、时间和温度之间未发现显著规律。
