伊朗人群中HLA - C等位基因的PCR - SSOP分子分型

PCR-SSOP molecular typing of HLA-C alleles in an Iranian population.

作者信息

Buhler S, Sanchez-Mazas A, Zanone R, Djavad N, Tiercy J-M

机构信息

Laboratory of Genetics and Biometry, University of Geneva, Switzerland.

出版信息

Tissue Antigens. 2002 Jun;59(6):525-30. doi: 10.1034/j.1399-0039.2002.590611.x.

DOI:10.1034/j.1399-0039.2002.590611.x

PMID:12445324

Abstract

HLA-C alleles were characterized by a polymerase chain reaction-sequence specific oligonucleotide probe (PCR-SSOP) hybridization protocol in a sample of 120 Iranians from Tehran. A total of 23 alleles were identified with the four most predominant--Cw0401, Cw0602, Cw1202, and Cw0701/06--accounting for almost 50% of HLA-C alleles. A comparison of HLA-C diversity among several populations indicates that Iranians stand at an intermediate genetic position between Europeans and Africans, an observation that may be related to their geographical location at a continental crossroads. The results also reveal a very high correlation between genetic and geographic distances on a global scale. A total of 30 HLA-C-DRB1 haplotypes were found in the Iranians, with the highest frequencies of 6.6% and 6.04 % being for Cw0602-DRB10701 and Cw1202-DRB11502, respectively.

摘要

采用聚合酶链反应-序列特异性寡核苷酸探针（PCR-SSOP）杂交方法，对来自德黑兰的120名伊朗人的样本进行HLA-C等位基因分型。共鉴定出23个等位基因，其中四个最主要的等位基因——Cw0401、Cw0602、Cw1202以及Cw0701/06——占HLA-C等位基因的近50%。对多个群体的HLA-C多样性进行比较后发现，伊朗人在基因上处于欧洲人和非洲人之间的中间位置，这一观察结果可能与他们处于大陆十字路口的地理位置有关。研究结果还揭示，在全球范围内，基因距离与地理距离之间存在非常高的相关性。在伊朗人中总共发现了30种HLA-C-DRB1单倍型，其中Cw0602-DRB10701和Cw1202-DRB11502的频率最高，分别为6.6%和6.04%。