Sun Jianzhong, Fuxa James R, Henderson Gregg
Department of Entomology, Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.
J Invertebr Pathol. 2002 Oct;81(2):78-85. doi: 10.1016/s0022-2011(02)00152-0.
The sporulation of 22 total isolates of Metarhizium anisopliae and Beauveria bassiana was quantified on cadavers of the Formosan subterranean termite, Coptotermes formosanus. Conidial production increased significantly over 11 days post-death. Effects of isolates of M. anisopliae and B. bassiana on in vivo sporulation were significant. Although the overall effects of fungal species on in vivo sporulation were not significant, the interactions between fungal species and certain times post-death were significant, indicating different sporulation patterns between the two fungal species. B. bassiana isolates could be categorized into a group with high total sporulation (day 11) and low quick sporulation (on days 2 and 3), while M. anisopliae isolates fell into another group with high quick sporulation and low total sporulation. This could give M. anisopliae an advantage over B. bassiana in termite microbial control due to termite defensive social behaviors. Conidial production was significantly higher in vitro than in vivo. In vitro and in vivo sporulation differed by as much as 89x and 232x among the selected isolates of B. bassiana and M. anisopliae, respectively. Correlation between in vivo and in vitro conidial production was positive and significant. This may allow preliminary in vitro screening of a large number of isolates for high in vivo sporulation.
在台湾乳白蚁（Coptotermes formosanus）的尸体上对22株绿僵菌（Metarhizium anisopliae）和球孢白僵菌（Beauveria bassiana）的总分离株的产孢情况进行了定量分析。死亡后11天内，分生孢子产量显著增加。绿僵菌和球孢白僵菌分离株对体内产孢的影响显著。虽然真菌种类对体内产孢的总体影响不显著，但真菌种类与死亡后特定时间之间的相互作用显著，表明两种真菌的产孢模式不同。球孢白僵菌分离株可分为总产孢量高（第11天）和快速产孢量低（第2天和第3天）的一组，而绿僵菌分离株则属于快速产孢量高和总产孢量低的另一组。由于白蚁的防御性社会行为，这可能使绿僵菌在白蚁微生物防治方面比球孢白僵菌具有优势。分生孢子产量在体外显著高于体内。在所选择的球孢白僵菌和绿僵菌分离株中，体外和体内产孢量的差异分别高达89倍和232倍。体内和体外分生孢子产量之间的相关性呈正且显著。这可能允许对大量分离株进行初步体外筛选以获得高体内产孢量。
