Ratnoff O D, Saito H
Blood. 1976 Feb;47(2):243-51.
Fitzgerald trait, an asymptomatic disorder, is associated with abnormalities of surface-mediated plasma reactions, including coagulation via the intrinsic pathway, augmentation of the clot-promoting properties of factor VII, kaolin-mediated fibrinolysis, kinin generation, and enhancement of vascular permeability by diluted plasma (PF/Dil). These abnormalities can be corrected by Fitzgerald factor, an agent probably identical with high molecular weight kininogen found in normal, but not Fitzgerald-trait plasma. Our preparations of Fitzgerald factor possessed a second property. Amidolysis of alpha-N-benzoyl-L-proline-L-phenylalanine-L-arginine-pnitroanilide by a plasma kallikrein (activated Fletcher factor) was inhibited by kaolin or solutions of ellagic acid. Addition of preparations of Fitzgerald factor to kaolin or to solutions of ellagic acid counteracted their inhibitory properties. The action of these preparations was duplicated by solutions of cytochrome C or IgG, suggesting that these agents may inhibit the negative charges of kaolin or ellagic acid. Fitzgerald factor enhanced amidolysis of both normal and Fitzgerald-trait plasmas exposed to kaolin, effects not duplicated by cytochrome C or IgG. Whether or not the two properties of our preparations of Fitzgerald factor are related to the same agent is not yet certain. The relationship between these observations and the biologic role of Fitzgerald factor remains to be investigated.
菲茨杰拉德特性是一种无症状疾病，与表面介导的血浆反应异常有关，包括通过内源性途径的凝血、因子VII促凝特性的增强、高岭土介导的纤维蛋白溶解、激肽生成以及稀释血浆（PF/ Dil）引起的血管通透性增强。这些异常可通过菲茨杰拉德因子纠正，该因子可能与正常血浆而非菲茨杰拉德特性血浆中发现的高分子量激肽原相同。我们制备的菲茨杰拉德因子具有第二种特性。血浆激肽释放酶（活化的弗莱彻因子）对α-N-苯甲酰-L-脯氨酸-L-苯丙氨酸-L-精氨酸-对硝基苯胺的酰胺水解作用受到高岭土或鞣花酸溶液的抑制。向高岭土或鞣花酸溶液中添加菲茨杰拉德因子制剂可抵消它们的抑制特性。细胞色素C溶液或IgG溶液可重复这些制剂的作用，表明这些试剂可能抑制高岭土或鞣花酸的负电荷。菲茨杰拉德因子增强了暴露于高岭土的正常血浆和菲茨杰拉德特性血浆的酰胺水解作用，细胞色素C或IgG无法重复此效果。我们制备的菲茨杰拉德因子的这两种特性是否与同一种试剂有关尚不确定。这些观察结果与菲茨杰拉德因子的生物学作用之间的关系仍有待研究。
