Kim Gyoung Nyoun, Choi Woo-young, Park Manhoon, Kang C Yong
Department of Microbiology and Immunology, Faculty of Medicine and Dentistry, Siebens-Drake Research Institute, University of Western Ontario, London Ont, Canada N6G 2V4.
Virus Res. 2002 Dec;90(1-2):347-64. doi: 10.1016/s0168-1702(02)00255-1.
The large (L) protein of vesicular stomatitis virus (VSV), catalytic subunit of RNA-dependent RNA polymerase is responsible for the transcription and replication of VSV. The L protein of the Indiana serotype of VSV (VSV(Ind)) has previously been cloned and expressed, and used in the reverse genetics of VSV(Ind). However, the cDNA clones expressing functional L proteins of the VSV(NJ) serotype were not available. It was necessary to obtain functional clones of the New Jersey serotype of VSV (VSV(NJ)) in order to study homologous viral interference. Here we report the cDNA cloning, expression, and functional analyses of L proteins from both the Hazelhurst subtype and Concan subtype of VSV(NJ). The analysis of the expressed L proteins for the transcription and replication of VSV demonstrate that both VSV(NJ) L clones express functional RNA-dependent RNA polymerase.
水泡性口炎病毒（VSV）的大（L）蛋白是RNA依赖性RNA聚合酶的催化亚基，负责VSV的转录和复制。VSV印第安纳血清型（VSV(Ind)）的L蛋白此前已被克隆和表达，并用于VSV(Ind)的反向遗传学研究。然而，尚无表达VSV新泽西血清型（VSV(NJ)）功能性L蛋白的cDNA克隆。为了研究同源病毒干扰，有必要获得VSV新泽西血清型（VSV(NJ)）的功能性克隆。在此，我们报告了VSV(NJ)哈泽赫斯特亚型和康坎亚型L蛋白的cDNA克隆、表达及功能分析。对所表达的L蛋白进行VSV转录和复制分析表明，两个VSV(NJ) L克隆均表达功能性RNA依赖性RNA聚合酶。
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