Revanasiddappa H D, Kiran Kumar T N
Department of Chemistry, University of Mysore, Manasagangothri, Mysore-570 006, India.
Anal Bioanal Chem. 2002 Nov;374(6):1121-4. doi: 10.1007/s00216-002-1581-7. Epub 2002 Oct 24.
A simple, rapid, and sensitive spectrophotometric method has been developed for the determination of selenium in real samples of water, soil, plant materials, human hair, and synthetic cosmetic and in pharmaceutical preparations. The method is based on the reaction of selenium with potassium iodide in an acidic medium to liberate iodine. The liberated iodine bleaches the violet color of thionin, and which is measured at 600 nm. This decrease in absorbance is directly proportional to selenium concentration and obeys Beer's law in the range 1-5 micro g selenium in a final volume of 10 mL (0.1-0.5 microg mL(-1)). The molar absorptivity and Sandell's sensitivity of the method were found to be 7.33 x 10(4) L mol(-1) cm(-1) and 0.0011 microg cm(-2), respectively. The optimum reaction conditions and other analytical conditions were evaluated. The effect of interfering ions on the determination is described.
已开发出一种简单、快速且灵敏的分光光度法，用于测定水、土壤、植物材料、人发、合成化妆品及药物制剂等实际样品中的硒。该方法基于在酸性介质中硒与碘化钾反应释放出碘。释放出的碘使硫堇的紫色褪去，并在600nm处进行测定。吸光度的降低与硒浓度成正比，在最终体积为10mL（0.1 - 0.5μg mL⁻¹）时，1 - 5μg硒的范围内符合比尔定律。该方法的摩尔吸光系数和桑德尔灵敏度分别为7.33×10⁴ L mol⁻¹ cm⁻¹和0.0011μg cm⁻²。评估了最佳反应条件及其他分析条件。描述了干扰离子对测定的影响。
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