Tseng R Y, Cohen N L, Reyes P S, Briggs G M
J Nutr. 1976 Jan;106(1):77-85. doi: 10.1093/jn/106.1.77.
Various metabolic changes were observed in male hamsters fed vitamin B-12-deficient diets with or without supplements of cobalt, methionine, and a previously untested cobalt-free pseudovitamin B-12. The effects observed after 31 weeks of consuming the vitamin B-12-deficient diets included a marked increase in the urinary excretion of both methylmalonic acid and formiminoglutamic acid, slight increases in red blood cell mean corpuscular volume, and higher tissue levels of glutathione and activities of glutathione reductase and glucose-6-phosphate dehydrogenase. Vitamin B-12 in the diet prevented these changes, as did inorganic cobalt. The cobalt-free pseudovitamin B-12 showed no vitamin B-12 activity, neither did it have any potent antagonistic effect. Methionine supplementation reversed some of the metabolic changes. Addition of inorganic cobalt to the diet resulted in a significant increase in tissue stores of vitamin B-12.
在喂食缺乏维生素B-12饮食的雄性仓鼠中，观察到了各种代谢变化，这些饮食添加或未添加钴、蛋氨酸以及一种之前未测试过的无钴假维生素B-12。食用缺乏维生素B-12饮食31周后观察到的影响包括甲基丙二酸和亚胺甲基谷氨酸的尿排泄显著增加、红细胞平均体积略有增加，以及谷胱甘肽组织水平升高和谷胱甘肽还原酶及葡萄糖-6-磷酸脱氢酶活性升高。饮食中的维生素B-12可预防这些变化，无机钴也有同样效果。无钴假维生素B-12未表现出维生素B-12活性，也没有任何显著的拮抗作用。补充蛋氨酸逆转了一些代谢变化。在饮食中添加无机钴导致维生素B-12的组织储存显著增加。
