Silvagno Francesca, Guarnieri Vincenzo, Capizzi Anna, Pescarmona Gian Piero
Dipartimento di Genetica, Biologia e Biochimica, Facolta' di Medicina e Chirurgia, Universita' di Torino, Sezione di Biochimica, Via Santena 5 bis, 10126 Turin, Italy.
FEBS Lett. 2002 Dec 4;532(1-2):153-8. doi: 10.1016/s0014-5793(02)03667-0.
Retinoic acid (RA) affects many cell types by either promoting their survival or inducing their differentiation. Dehydroepiandrosterone (DHEA), a precursor for both androgenic and estrogenic steroids and abundantly produced by brain, is known as an inhibitor of cell proliferation. Differentiation of a human neuroblastoma cell line (SK-N-BE) was evaluated measuring growth rate, motility, neurite extension and GAP-43 expression. We report that DHEA enhances the differentiating effect of RA on neuroblastoma cells via a signalling that is not RA receptor-mediated. Instead, we show a differential expression of matrix metalloproteinases: RA enhances the activity of MMP-2, whereas MMP-9 expression is up-regulated by DHEA. The concerted modulation of these proteinases may support the neurite outgrowth observed after co-treatment with the two drugs.
视黄酸（RA）通过促进多种细胞类型的存活或诱导其分化来影响它们。脱氢表雄酮（DHEA）是雄激素和雌激素类固醇的前体，由大脑大量产生，是一种已知的细胞增殖抑制剂。通过测量生长速率、运动性、神经突延伸和GAP - 43表达来评估人神经母细胞瘤细胞系（SK - N - BE）的分化。我们报告称，DHEA通过一种非RA受体介导的信号增强RA对神经母细胞瘤细胞的分化作用。相反，我们发现基质金属蛋白酶的差异表达：RA增强MMP - 2的活性，而DHEA上调MMP - 9的表达。这些蛋白酶的协同调节可能支持两种药物联合处理后观察到的神经突生长。
