噬菌体T7 RNA聚合酶的结构功能分析

Structural-functional analysis of bacteriophage T7 RNA polymerase.

作者信息

Tunitskaya V L, Kochetkov S N

机构信息

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.

出版信息

Biochemistry (Mosc). 2002 Oct;67(10):1124-35. doi: 10.1023/a:1020911223250.

DOI:10.1023/a:1020911223250

PMID:12460110

Abstract

This review summarizes our results of the structural and functional studies of bacteriophage T7 DNA-dependent RNA polymerase (T7 RNAP). Particular features of this enzyme (the single-subunit composition, relatively low molecular weight) make it the most convenient model for investigating the physicochemical aspects of transcription. The review discusses the main properties of T7 RNAP, interaction between the enzyme and promoter, principle stages of T7-transcription, and also the results of structural and functional studies by affinity modification and both random and site-directed mutagenesis techniques.

摘要

本综述总结了我们对噬菌体T7 DNA依赖性RNA聚合酶（T7 RNAP）进行结构和功能研究的结果。该酶的独特特性（单亚基组成、相对较低的分子量）使其成为研究转录物理化学方面最便捷的模型。综述讨论了T7 RNAP的主要特性、酶与启动子之间的相互作用、T7转录的主要阶段，以及通过亲和修饰、随机诱变和定点诱变技术进行结构和功能研究的结果。