一种新型人类VMP基因的克隆、表达及特性分析

Cloning, expression and characterization of a novel human VMP gene.

作者信息

Cheng Chao, Xu Jian, Ye Xin, Dai Jianfeng, Wu Qihan, Zeng Li, Wang Liu, Zhao Wei, Ji Chaoneng, Gu Shaohua, Xie Yi, Mao Yumin

出版信息

Mol Biol Rep. 2002 Sep;29(3):281-6. doi: 10.1023/a:1020402410522.

DOI:10.1023/a:1020402410522

PMID:12463420

Abstract

We report here cloning and characterization of a novel human gene, termed VMP, which is a vesicular membrane protein. RT-PCR analysis shows that VMP is expressed exclusively in brain of the 16 tissues examined, suggesting that it is a neuron-specific membrane protein. The cDNA encodes 195 amino acid with a putative molecular weight of about 24 KDa. VMP contains two putative membrane spanning domains and a hydrophilic tail homologous to the microtubule-binding domain of MAPs. So it is speculated that VMP may associated with microtubules through its C-terminal and plays an important role in vesicular organelles transport and nerve signals.

摘要

我们在此报告一个名为VMP的新型人类基因的克隆与特性分析，它是一种囊泡膜蛋白。逆转录聚合酶链反应（RT-PCR）分析表明，在所检测的16种组织中，VMP仅在大脑中表达，这表明它是一种神经元特异性膜蛋白。该互补DNA（cDNA）编码195个氨基酸，推测分子量约为24千道尔顿。VMP包含两个推测的跨膜结构域和一个与微管相关蛋白（MAPs）的微管结合结构域同源的亲水尾部。因此推测VMP可能通过其C末端与微管相关，并在囊泡细胞器运输和神经信号传导中发挥重要作用。