用于鸟类分子性别鉴定的限制性酶切位点DdeI在德氏冕鹤中缺失。

Loss of restriction site DdeI, used for avian molecular sexing, in Oreophasis derbianus.

作者信息

Bermúdez-Humarán L G, Chávez-Zamarripa P, Guzmán-Velasco A, Leal-Garza C H, Montes de Oca-Luna R

机构信息

Laboratorio de Inmunología y Virología, Facultad de Ciencias; Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza N.L. México.

出版信息

Reprod Domest Anim. 2002 Dec;37(6):321-3. doi: 10.1046/j.1439-0531.2002.00362.x.

DOI:10.1046/j.1439-0531.2002.00362.x

PMID:12464068

Abstract

Molecular sexing is a rapid and safe procedure for bird sex determination. Two universal methods based on the amplification of a chromo-helicase-DNA-Binding 1 (CHD) gene region, located in both sexual chromosomes (Z and W), have been established. We found that molecular sexing of Oreophasis derbianus failed by using these two procedures. One of them is based on a restriction site located in CHD1W gene but absent in CHD1Z. The DdeI restriction site, used successfully to determine gender in several bird species, was found to be lost because of nucleotide change in O. derbianus. This change created a new restriction site, NlaIII, that was successfully applied for sexing this endangered bird.

摘要

分子性别鉴定是一种快速且安全的鸟类性别确定方法。基于对位于两条性染色体（Z和W）上的染色体解旋酶DNA结合蛋白1（CHD）基因区域进行扩增，已建立了两种通用方法。我们发现，使用这两种方法对德氏凤冠雉进行分子性别鉴定均失败。其中一种方法基于CHD1W基因中存在但CHD1Z基因中不存在的一个限制性酶切位点。在几种鸟类中成功用于确定性别的DdeI限制性酶切位点，因德氏凤冠雉核苷酸变化而丢失。这种变化产生了一个新的限制性酶切位点NlaIII，该位点已成功用于对这种濒危鸟类进行性别鉴定。